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    Preliminary Characterization of Three Populations of the Freshwater Sponge, Clypeatula cooperensis, using RAPD-PCR

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    2000_WilliamsK_THS_000209.pdf (1.356Mb)
    Author
    Williams, Kathryn
    Advisor
    John Addis; Linda Beischel; Gerald Shields
    Date of Issue
    2000-04-01
    Subject Keywords
    freshwater sponge, Clypeatula cooperensis
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/2821
    Title
    Preliminary Characterization of Three Populations of the Freshwater Sponge, Clypeatula cooperensis, using RAPD-PCR
    Type
    thesis
    Abstract
    In order to relate the three known populations of the freshwater sponge, Clypeatula cooperensis, to one another, sponge DNA was subjected to RAPDPCR. DNA was extracted using a Puregene kit from three specimens collected from each population. Following PCR optimization, DNA was amplified using random primers (OPA-10, 11, 12, 13, 18, and 20). Although reproducibility was a problem, preliminary results suggest that variability within populations is as high as variability among populations, raising the possibility that the three populations are genetically identical. This is an unexpected finding because one population is geographically isolated from the other two. If correct, one explanation is that the three populations have only recently become separated.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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