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    Microsatellite Analysis of Gene Flow between Culex tarsalis Populations Connected by Irrigation in Yellowstone County, Montana

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    CaseyM_2013.pdf (588.8Kb)
    Author
    Casey, Mattie
    Advisor
    Jennifer Gloweinka; Grant Hokit; Holly Zullo
    Date of Issue
    2013-04-01
    Subject Keywords
    Culex tarsalis, mosquitoes, West Nile virus
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/2782
    Title
    Microsatellite Analysis of Gene Flow between Culex tarsalis Populations Connected by Irrigation in Yellowstone County, Montana
    Type
    thesis
    Abstract
    The purpose of this study was to determine whether population connectivity by irrigation enhances gene flow between Culex tarsalis populations in the state of Montana. Four populations of Cx. tarsalis were collected along the Yellowstone and Bighorn rivers and allele frequencies obtained from PCR amplification of four microsatellite loci, visualized using the QIAxcel Advanced System, were used to assess the genetic structure of the populations. Results of the four loci indicate lower pairwise FST values between only two of three populations connected through waterway, which suggests a disparity in the data. If the genetic similarity between these populations reflects rates of gene flow, these results suggest that higher degrees of gene flow may not be due to irrigation but rather to connectivity by any waterway. Further analysis of additional polymorphic microsatellite loci needs to be performed to determine if a higher degree of gene flow does indeed occur between populations connected by irrigation.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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