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    Isolation and Analysis of the Novel Mycobacteriophage, CCP1

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    McNameeK_2015_Final.pdf (1.152Mb)
    Author
    McNamee, Kevin
    Date of Issue
    2015-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/2767
    Title
    Isolation and Analysis of the Novel Mycobacteriophage, CCP1
    Type
    thesis
    Abstract
    Bacteriophages are the largest remaining body of undescribed biodiversity on the planet. The present study attempted to isolate and analyze the structure and genetics of a novel mycobacteriophage extracted from a soil sample. It was hypothesized that undescribed phages exist in the study area. A phage, named CCP1, was isolated from soil obtained in Helena, Montana, U.S.A. via serial plating of phage material taken from plaques formed in Mycobacterium smegmatis. Genetic and morphological analyses by restriction enzyme fragment length polymorphism (RFLP) analysis and transmission electron microscopy (TEM) respectively, suggest that CCP1 is a novel mycobacteriophage.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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