Metabolic Profiles of Synovial Fluid and Platelet-Poor Plasma by Osteoarthritic Grade
Osteoarthritis (OA) is a chronic degenerative synovial joint and bone disease characterized by articular cartilage degradation and affecting nearly 654.1 million adults worldwide. It is currently diagnosed and graded using radiologic imaging, which primarily relies on physicians' subjective interpretations of the radiographs. This is at times inconsistent across the medical field and proves problematic in accurately assessing the state of disease. The primary aim of this study is to compare the metabolic profiles of synovial fluid (SF) and platelet poor plasma across different grades of OA in order to identify biomarkers that can be used to accurately ascertain the severity of OA. Understanding how local (SF) and systemic (blood plasma) responses to stress vary on a metabolic level could aid in developing a form of plasma based diagnosis that is more objective than radiography and less invasive than acquiring synovial fluid. Detecting biomarkers in blood plasma allows for quick metabolic analysis that can be used to diagnose OA in its earlier stages, leading to a positive patient prognosis. Early and late OA (n=2) synovial fluids will be analyzed by liquid chromatography mass spectrometry (LC-MS) to measure global metabolic changes. Platelet poor plasma from individuals with early and late OA (n=2) will be assessed as well. Comparisons between early OA (grades II-III), and late OA (grades III+) synovial fluids and blood plasma samples will be analyzed to assess characteristics of each stage of disease progression both locally and systemically.