Studies Towards the Synthesis of Novel Oxadiazole Derivatives for Antibiotic Screening Against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.
Undergraduate work by Michael Henderson and Patrick Hewes to synthesize a novel diazole antibiotic, and to standardize Kirby Bauer and MIC assays using the tools and resources available at Carroll College.
Bacterial antibiotic resistance continues to render current pharmaceutical drugs ineffective at preventing the growth of hard-to-treat bacterial strains. Persistent under current treatments, methicillin-resistant Staphylococcus aureus (MRSA) has presented as a major issue on a global level, impacting increasing numbers of patients in clinical settings (O’Daniel, et. al).1 Hypothesis: Treatment of an oxadiazole class and selected control antibiotics to bacterial strains will exhibit a significant drop in growth. Our approach: Determination of antibacterial properties-effective concentrations was achieved by Kirby-Bauer disk diffusion and Minimum Inhibition Concentration assay(s) (MIC). Results: We report an unsuccessful attempt to synthesize a novel oxadiazole antibiotic, but present promise in standardizing biological assays using three control antibiotics. In conclusion: the data indicates that Penicillin, Vancomycin, and Gentamicin display antibacterial properties against the growth of Escherichia-coli, Staphylococcus aureus, and Pseudomonas aeruginosa. We speculate, with this report, further antibiotic classification at Carroll College is possible with the standardized procedure, once novel antibiotics are synthesized.