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    Molecular Diversity of West Nile Virus in Montana

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    Author
    Sciuchetti, Julia
    Advisor
    Glowienka, Jennifer; Greiner, Katherine; Hokit, David
    Date of Issue
    2020
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/10287
    Title
    Molecular Diversity of West Nile Virus in Montana
    Type
    Thesis
    Abstract
    West Nile virus (WNV) has been present in the United States since 1999 and like many RNA viruses, it is known to have high mutation rates. At Carroll College, a decade long project continues in which the state of Montana is surveyed for the presence of WNV. We have created and continue to refine a risk assessment map to predict where outbreaks will occur in a given year. The goal of this particular study was to assess and compare genetic variation of West Nile virus sequences found within geographically distinct mosquito populations across the state of Montana using the highly variable env gene. Two different primer sets were utilized to perform reverse transcription and DNA amplification on the viral RNA, which was followed by gel electrophoresis to visualize the amplifications. Amplification was achieved in a subset of samples and the products were sequenced, although the resulting sequences were of low quality. Knowledge about the evolution and diversification of the WNV in Montana will contribute to refinement of the risk assessment model, which should help in making better predictions of WNV outbreaks.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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