Browsing by Author "Otto-Hitt, Stefanie"
Now showing items 1-20 of 25
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Analysis of FBP1 Gene Expression in UV Light-exposed Tetrahymena thermophila Cultures
Henry, Colby; Otto-Hitt, Stefanie (2018-07-01)In this study, Tetrahymena thermophila cultures were exposed to an acute treatment of UV light and expression of the FBP1 gene was examined. The FBP1 gene encodes Fructose- 1,6-biphosphatase which plays an important role ... -
The Analysis of the Ebony Gene in Culex tarsalis Across the Major Drainage Basins of Montana
Antonioli, Nathaniel (2022)Many diseases are transmitted by vectors one of which is West Nile Virus (WNV). WNV has been in the United States since 1999 and Montana since 2002. The main vector for WNV in Montana is Culex tarsalis. Carroll College ... -
Analyzing ATP Synthetase F1 Expression in Heat-Exposed Tetrahymena thermophila
Gardner, Kevin; Lorang, Ian; Otto-Hitt, Stefanie (2018-07-01)In this study, we investigated the effect of temperature on expression of the ATP1 gene in the organism Tetrahymena thermophila. We hypothesized that expression of the ATP1 gene would increase as we increased temperature. ... -
Assessing the Global Metabolic Response of Encapsulated Chondrocytes to Simulated Microgravity
Cooper, Gwendolyn (2021)Space sojourns are crucial for understanding aeronautics, physics, the solar system, and evaluating the possibility of interplanetary travel. However, there are many potential health risks associated with space travel such ... -
Effect of Alcohol on PFK1 Gene Expression and Feeding Activity in Tetrahymena thermophila
Sowers, Evelyn; Skoulis, Alex; Otto-Hitt, Stefanie (2018-07-01)The purpose of this experiment was to test whether alcohol induces starvation conditions in Tetrahymena thermophila. For this experiment, it was hypothesized that alcohol induces starvation due to a lack of energy available ... -
Effect of EGTA on SIT1 Scramblase Gene Expression and Cell Growth in Tetrahymena thermophila
Esposito, Emma; Knauth, Theda; Ohnstad, Amelia; Otto-Hitt, Stefanie (2018-07-01)Scramblase is an enzyme that facilitates the movement of newly synthesized phospholipids from the cytosolic side to the extracellular side of the lipid bilayer. This process is vital for cell membrane repair and growth. ... -
Effect of Nitrate Pollution on Tetrahymena thermophila
DeBruycker, Reegan; Sylvester, Hannah; Otto-Hitt, Stefanie (2018-07-01)The question under investigation in this project was: Does an elevated concentration of nitrates have a negative eect on cell growth and expression of the CDC16 gene in the organism Tetrahymena thermophila? Exposure of ... -
Effect of Potassium Bromate on OXR1 Gene Expression and Cell Growth in Tetrahymena thermophila
Khale, Scott; McMahon, Brendan; Otto-Hitt, Stefanie (2018-07-01)The purpose of our experiment was to answer the question: Does oxidative damage, induced by potassium bromate (KBrO3), affect growth rate and expression of the OXR1 gene in Tetrahymena thermophila? Potassium Bromate was ... -
Effects of Fatty Acids from Avocados on Expression of the HMG1 Gene in Tetrahymena thermophila
Phan, Jasmine; Kane, Connor; Otto-Hitt, Stefanie (2018-07-01)The enzyme 3-Hydroxy-3-Methyl-Glutaryl-CoA Reductase (HMG) HMG catalyzes the ratelimiting step in the biosynthesis of cholesterol and is a common drug target for cholesterol reduction. Previous studies in rats have shown ... -
Effects of Salt Concentration on Tetrahymena thermophila Growth and CRP1 Gene Expression
Mchugh, Katie; Tremaine, Rachel; Sapone, Anna; Otto-Hitt, Stefanie (2018-07-01)The use of salt to remove ice from roads has resulted in increased salinity in many freshwater lakes in North America. For this project, we wanted to explore how environmental salt concentrations influence the rate of ... -
Effects of Sulfur Dioxide on abdA Gene Expression and Larval Development in Drosophila melanogaster
Esposito, Emma; McNally, Keenan; Otto-Hitt, Stefanie (2018-07-01)In this experiment, we tested the effects sulfur dioxide exposure had on abd-A gene expression and larval development in Drosophila melanogaster. Previous studies showed that sulfur dioxide exposure inhibits development ... -
Effects of Tartrazine on Drosophila melanogaster Anxiety and Depression Behaviors
Unterseher, Chloe; Tupper, Francie (2022)Tartrazine is a yellow-tinged chemical that is used to dye food, fabric, drugs, and cosmetics yellow in the United States. Previous research has found a connection between tartrazine-rich diets and anxiety and depressive ... -
Effects of the Everyday Toxin, Titanium Dioxide, on Drosophila melanogaster Nervous System Development
Carparelli, Maria; Ripley, Kelsey; Otto-Hitt, Stefanie (2018-07-01)Titanium dioxide (TiO2) is a widely used compound found in everything from food packaging to sunscreens. When ingested, TiO2 is readily transported across membranes and efficiently stored within cells. Previous studies ... -
Effects of UVB light on Tfb5 gene expression in developing Drosophila melanogaster
Bullick, Teal; Green, Bryce; Otto-Hitt, Stefanie (2018-07-01)UVB light is known to cause DNA damage that results in gene mutations. Most regions of DNA damage are corrected using Nucleotide Excision Repair mechanisms (NER). In Drosophila melanogaster, the Tfb5 gene codes for an ... -
Exploring the Effects of Environmental Temperature on CDC-7 Expression in T. thermophila
Brothers, John; Gerry, Alexis; Scofield, Lauren; Otto-Hitt, Stefanie (2018-07-01)Tetrahymena thermophila are unicellular eukaryotes with a remarkably large genome: over 27,000 genes. One important aspect of studying the Tetrahymena genome is identifying and understanding what factors influence gene ... -
Impacts of Environmental Motion on CDC2 Expression and Cell Growth in Tetrahymena thermophila
Rees, Jack; Brady, Keaton; Otto-Hitt, Stefanie (2018-07-01)In this experiment, the eect of constant motion on expression of the cell cycle control gene CDC2 was examined in the organism Tetrahymena thermophila. We hypothesized that CDC2 expression would decrease with a corresponding ... -
Investigation of Nicotine’s Effect on Drosophila Melanogaster Motor Function and Aggression
Nelson, Carrie; Normandeau, James (2022)Nicotine, a highly addictive chemical, is a common component of e-cigarette juice. Given e-cigarette popularity among teenagers and young adults, we decided to investigate the link between nicotine and behavior. Our project ... -
“I’m Here to Understand You”: The Roles of the First, Second, and Third-Person Perspectives in Empathy
Breit, Julianna (2023)The project of empathy marks a long-standing debate between Theory of Mind defenders and phenomenologists. While they differ on their mechanisms of empathy, their research generally circles around the same question, “Do ... -
Manganese Toxicity in the Dopamine Synthesis Pathway in Drosophila melanogaster
Adair, Brandon; Jones, Tamra; Otto-Hitt, Stefanie (2018-07-01)Manganese is prevalent in Montana, and Manganese toxicity symptomatically resembles Parkinsons disease. The goal of this project was to determine whether exposure to high levels of Manganese affects the Dopamine synthesis ... -
Put Your Phone Down: Effects of Cellular Radiation on Drosophila melanogaster
Sapone, Anna; Wilson, Michael; Otto-Hitt, Stefanie (2018-07-01)In this project, we aimed to answer the question: Does cellular radiation affect offspring production and expression of the Rad51 gene in the organism Drosophila melanogaster? It was hypothesized that if Drosophila larvae ...